TB-500 10mg User Guide

This guide is for standalone TB-500 10mg. It covers reconstitution, dosing, subQ injection basics, side effects, and storage.

You can find this product here: TB-500.

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ABOUT THIS PEPTIDE

TB-500 is a synthetic fragment related to thymosin beta-4. It is usually discussed around mobility, soft-tissue repair, flexibility, range of motion, and recovery from training wear and tear.

BPC-157 gets more attention for localized tendon, ligament, and gut discussions. TB-500 is usually described as the broader, more systemic recovery peptide. People often pair them, but standalone TB-500 allows a less frequent schedule and independent dose control.

Community protocols commonly use a larger dose once or twice per week. That convention does not come from controlled human trials. The FDA reported that it could not find human exposure studies for TB-500 by any route, so human safety and effective dosing remain unknown.

TB-500 is prohibited for tested athletes.

Quick notes:

• Best known for: mobility, soft-tissue, and systemic recovery discussions.
• Evidence level: preclinical thymosin beta-4 biology, no established human TB-500 dose.
• Community theme: 2-2.5mg twice weekly during a short loading block.
• Main watch-out: confusing TB-500 with full-length pharmaceutical thymosin beta-4 research.

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SUPPLIES YOU’LL NEED

• Insulin needles, https://www.amazon.com/dp/B07P2KK5P7
• Alcohol swabs, https://www.amazon.com/dp/B07F2MQ9NJ
• Reconstitution solution (bacteriostatic water), https://progressionpeptides.com/product/bacteriostatic-water/

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RECONSTITUTION (MIXING)

Each vial contains 10mg of TB-500. Add 2mL of bacteriostatic water.

1. Draw up 200 units of bacteriostatic water in total.
2. Add it slowly down the inside wall of the vial.
3. Swirl gently until dissolved. Do not shake.

With this mix, 1 unit = 0.05mg, or 50mcg, of TB-500.

Units on syringe	TB-500 dose
10 units	0.5mg
20 units	1mg
40 units	2mg
50 units	2.5mg

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DOSING SCHEDULE

Simple starting protocol

• First week: 20 units (1mg), twice during the week.
• If tolerated: 40 units (2mg), twice weekly with 3-4 days between doses.
• Run for 4-6 weeks, then stop and reassess.

Some community protocols use 50 units (2.5mg) twice weekly, but the evidence does not establish that 5mg per week works better or is safe. Start lower.

TB-500 is generally treated as systemic. Do not inject into a tendon, joint, wound, or painful structure.

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HOW TO INJECT

Watch this quick video for the basic subQ process: https://www.youtube.com/watch?v=e9NGueNM03I

1. Wipe the vial top with an alcohol swab.
2. Draw the dose.
3. Pinch a small area of belly or thigh fat and inject slowly.
4. Rotate sites.

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SIDE EFFECTS AND CAUTIONS

Human safety data are limited. Reported or possible effects include injection-site irritation, headache, fatigue, nausea, dizziness, flushing, sleep changes, or a temporary heavy feeling.

Use medical guidance with active cancer, bleeding or clotting disorders, blood-thinning medication, cardiovascular disease, pregnancy or breastfeeding, or an upcoming operation. Theoretical concern around new blood-vessel signaling is one reason to avoid casual use with active cancer.

Get medical care for chest pain, shortness of breath, one-sided leg swelling, fainting, severe headache, allergic swelling, or a serious injury.

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STORAGE

Keep the mixed vial refrigerated, cold, dry, and dark. Discard it if the liquid becomes cloudy, changes color, develops particles, or looks contaminated.

Write the mixing date on the vial. Aim to use it within 4 weeks and do not stretch it past 6 weeks.

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RESEARCH NOTES

• https://swolverine.com/en-au/blogs/blog/tb-500-dosage-guide-how-much-should-you-take-for-recovery-and-injury-repair
• https://www.fda.gov/media/193349/download
• https://www.reddit.com/r/Peptides/comments/15prnoa/