NAD+ 500mg User Guide

This guide is for NAD+ 500mg. NAD+ is not technically a peptide, but it is handled like one because it is supplied as lyophilized powder and reconstituted before subQ injection.

You can find this product here: NAD+.

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ABOUT THIS PEPTIDE

NAD+ is not a peptide. It is a core cellular coenzyme involved in energy metabolism, redox reactions, mitochondrial function, DNA repair signaling, and several aging-related pathways. In plain English, NAD+ helps cells move electrons and manage energy chemistry.

NAD+ gets so much attention because levels and related pathways change with age, metabolic stress, sleep disruption, alcohol use, illness, and hard training. That makes it popular in longevity, energy, recovery, and mitochondrial-support conversations. The biology is real, but the wellness marketing around NAD+ can get extremely loud.

The evidence is strongest for NAD+ biology and for precursor research, not for every claim made about injectable NAD+. Human outcome data is still mixed and depends heavily on route, dose, population, and what endpoint is being measured. Feeling flushed, heavy, tired, or uncomfortable during aggressive NAD+ protocols is common enough that conservative dosing matters.

Community reports vary widely. Some people describe clearer energy, better recovery, less brain fog, or improved resilience after a cycle. Others mostly feel the acute discomfort and not much benefit. The practical takeaway is that NAD+ belongs in the cellular-energy and longevity-support bucket, but it is not instant youth in a vial.

Quick notes:

• Best known for: cellular energy, mitochondrial, redox, recovery, and longevity research.
• Mechanism: coenzyme activity in redox and energy metabolism pathways.
• Evidence level: strong basic biology, mixed human wellness-outcome evidence.
• Community theme: energy/recovery/brain-fog reports, but response varies a lot.
• Main watch-out: dosing discomfort, flushing/heaviness, and anti-aging hype.

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SUPPLIES YOU’LL NEED

• Insulin needles - https://www.amazon.com/dp/B07P2KK5P7
• Alcohol swabs - https://www.amazon.com/dp/B07F2MQ9NJ
• Reconstitution solution (bacteriostatic water) - https://progressionpeptides.com/product/bacteriostatic-water/

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RECONSTITUTION (MIXING)

Add 5mL bacteriostatic water. This makes 1 unit = 1mg NAD+.

Units on syringe	Approx dose
10 units	10mg
20 units	20mg
25 units	25mg
50 units	50mg
75 units	75mg
100 units	100mg

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DOSING SCHEDULE

Start with 20-25mg 2-3 times per week. If tolerated, a simple working dose is 50mg 2-3 times per week. Higher doses such as 75-100mg are more aggressive and should be worked up to slowly.

NAD+ can sting and can cause a flush/rush feeling if pushed too high or injected too quickly. Inject slowly.

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HOW TO INJECT

Watch this quick video, it covers the basic subQ process: https://www.youtube.com/watch?v=e9NGueNM03I

The basics:

1. Wipe the top of the NAD+ bottle with an alcohol swab.
2. Draw your dose into the syringe.
3. Pinch a bit of belly fat or thigh, insert the needle, and push the plunger slowly.
4. Done. Rotate injection sites to avoid irritation.

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SIDE EFFECTS AND CAUTIONS

Possible side effects include injection-site burning, flushing, nausea, headache, fatigue, anxiety-like feelings, dizziness, or feeling generally off. Use extra caution with complex medical conditions, pregnancy/breastfeeding, active cancer history, methylation issues, or medication-heavy protocols.

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STORAGE

Keep the bottle in the fridge after reconstitution: cold, dry, and dark. Avoid repeated warm-ups, direct light, and unnecessary vial punctures.

Unreconstituted powder is usually more stable than mixed solution, but the fridge is still the better default. If the solution becomes cloudy, changes color, grows particles, or looks wrong, stop and do not use it.